The viral plaque assay is a method for the detection and quantitation of infectious cytopathic viruses which are added in different dilutions in a cell layer.
The difficulty is to find lysed cell areas between a confluent cell monolayer – the plaque center may miss out cells due to virus-induced lysis.
The high-resolution, precision optics of SYNENTEC’s cell imagers is very well suited for this type of virus analysis. For standardized and quick evaluation of this assay SYNENTEC developed the viral plaque operator.
Extract from the software readout (for further information look at ShortNote Plaque Assay)
• # of Plaques
• Plaque Area [%]
• Average Plaque size [µm2]