APPLICATION NOTE by SECURECELL
Process control is a central task in bioprocess development and production. Apart from common control tasks including pH, temperature or dissolved oxygen, more advanced control tasks must be mastered. Real-time availability of analytical data is often the basis to achieve a control based on process understanding. This application note demonstrates how sophisticated control tasks can be easily implemented in the Process Information Management Software Lucullus PIMS.
Exemplary, a temperature shift in an Escherichia coli (E. coli) process is automatically performed based on different on-line signals available in Lucullus PIMS: i) optical density and ii) glucose and lactose concentration determined by automated sampling and sample preparation via Numera followed by on-line HPLC analysis. Both approaches are compared with respect to the current state of the culture as well as their impact on process robustness..