Application Note by cytena

In most aerobic bioprocesses, oxygen deficiency occurs because aerobic organisms consume oxygen faster than the rate at which oxygen enters aqueous medium. Most conventional solutions to this limitation only apply to large-scale culturing. cytena’s study demonstrates that the c.bird system improves oxygen transfer in standard 96-well plates by allowing controllable mixing that increases oxygen transfer into aqueous medium. An extracellular oxygen assay was used to measure oxygen levels inside 96-well plates by comparing glucose oxidation after three different mixing conditions: static, c.bird 50-second and c.bird 25-second mixing periods. Results show that the c.bird significantly increased the oxygen transfer into the aqueous medium in 96-well plates, providing a better culturing environment for cells and other aerobic organisms.

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