Next generation microbioreactor for cell line development.
The innovative cytena c.bird microbioreactor technology – is the first microbioreactor that combines high-throughput culture screening, monitoring and optimisation from early stage of cell culture. It enables users to have better predictability of cell clones from day 1 and save weeks in the very cost intensive early-stage of the cell line development process. In addition, cell culture based research (e.g. synthetic biology, stem cell research and cell therapy) can potentially also benefit from c.bird technology.
Bringing production bioreactor conditions to 24-well and 96-well plates
The c.bird is a high-throughput microbioreactor for parallel cultivations in 24-well and 96-well plates that brings production bioreactor conditions to the 150 to 1600 µL scale.
Cutting weeks off the cell line development process
The c.bird enables early identification of the highest producing and most stable clones in order to reduce later passaging steps and efforts of current scaling-up process.
Better predictability of best cell clones
The c.bird screens and monitors valuable cell culture parameters for hundreds of cell lines from the earliest stages, helping scientists define the best clones sooner.
Optimise cell culture environments in 24-well and 96-well plates
- Increase oxygen transfer rate (OTR) for both adherent cells and suspension cells
- Bring cells into suspension culture (only for suspension cells)
- Continuous mixing for homogenous media composition
- Low shear rate mixing to reduce stress on cell lines
CHO-K1 mAb expressing cell line was used for the study. The cell line was adapted to the culture suspension in a chemically defined and animal-component-free medium (CD Hybridoma medium # 11279-023 by GibCo).
Standard 96-well plates (Eppendorf, Germany) were used for all experiments. Comparison studies were performed with cells cultivated in standard static culture and c.bird suspension culture in a 37°C, 5% CO2 incubator environment. Resulting cell growth and protein production were compared.
The c.bird suspension culture in 96-well plates improves cell proliferation, recombinant protein yield and volume-specific productivity (Qp) compared to traditional static culture. We investigated different initial cell concentrations and measurements were done using multiple instruments to ensure accuracy. In all cases, the c.bird with suspension culture exhibited better performance than static culture.
In case 1, the c.bird suspension culture achieved a cell density of 4.2×106 cells/ml, but the static culture only reached 1.8×106 cells/ml alter 3 days of culture. In case 2, c.bird suspension culture achieved a cell density of 3.1×106 cells/ml, but the static culture only reached 1.9×106 cells/ml alter 5 days of culture.
The c.bird also demonstrated -2.5-3.8 times higher recombinant protein yield than static culture. (Fig. 3). For the relative volume-specific productivity (Qp), c.bird suspension culture generated -1.8-2.6 times higher Qp than static culture.
For the relative volume-specific productivity (Qp), the c.bird suspension culture generated -1.8-2.6 times higher Qp than static culture.
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