Part 3: the S.NEST microbioreactor

Continuing to build the CLD lab of my dreams with the third item on my wish list – and it is a treat!

You may have seen that a shiny new microbioreactor has recently been launched by cytena Bioprocess Solutions, and like a moth to a flame – I must have it.

I am referring to the S.NEST microbioreactor. Before I get into what this device can do, let’s discuss where it fits in to my CLD workflow. Firstly, what is a microbioreactor? This is a device which simulates the conditions of larger downstream bioreactors on a much smaller scale. The idea here is to expose cells to suspension culture conditions earlier in the CLD process, to identify which cell lines might be the most robust and highest producing, and ultimately save time by reducing expansion steps. A suspension environment also provides more oxygen and growing space than static cultures, equating to a much better environment.

There are a few different microbioreactors on the market with a range of offerings depending on what you need, but the S.NEST is ideal because it is a combination of the best features, highlighted here:

pH and DO Sensing

This was a much-anticipated feature for this instrument, and is even the inspiration for the name (S – for Sensing). With optodes on the bottom of each well, pH and dissolved oxygen can be measured in real time.

Plate Capacity

This S.NEST has room for 4 plates (24 -well, 96 -well, or even a custom combination of the two).


A notable upgrade from the earlier model of this device, the c.bird, the S.NEST functions as an incubator, so no need to make room in your already packed laboratory incubator!

Unique Mixing Technology

cytena developed a reciprocated mixing technology which is different to others on the market. A redesigned conical shaped lid minimises the risk of evaporation, which is a common worry with microbioreactors.

Easy to Use

As with the single cell printers that I mentioned in my last blog, and all of cytena’s products, the S.NEST is easy to use. An uncomplicated instrument with sophisticated and yet easy to interpret analysis, the S.NEST was designed with the users in mind.

So to summarise my dream CLD lab so far, I’ll start by depositing single cells with my UP.SIGHT, I’ll grow my cells up, do some imaging and analysis using my NyONE imager, and then gather some data on which could be the best lines using my S.NEST microbioreactor. What should my next item be?

To discuss any of my recommendations please get in contact with me at – I would love to discuss your process with you.

Article by:
Ellen Harrington
Technical Sales Specialist


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