The main component of the CellSurgeon is a NIR femtosecond laser. The laser beam is coupled into a microscope and tightly focussed by high numerical aperture objectives. Inside the laser focus very high intensities occur, inducing non-linear optical processes and finally leading to ablation.
The low laser energies and the high numerical aperture objectives allow very precise manipulation and dissection on a subcellular level without harming adjacent structures. Depending on the configuration cuts with minimum dimensions of approx. 100 nm are possible. This gentle dissection method reduces the risk of cell death and is therefore especially suited for living cells.
The Rowiak CellSurgeon is a new laser nanodissection system enabling gentle and contact-free manipulation of living cells or subcellular structures. Applications include transfection of cells, deactivation of cell organelles and investigation of cell dynamics.
For more information please contact us on 01795 411 531 or visit www.rowiak.com
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